Difference between revisions of "Part:BBa K902041"
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| − | <html><i>AmdA</i> coding sequence (<a href="https://parts.igem.org/Part:BBa_K902035">K902035</a>) downstream of a strong TetR repressible promoter (<a href="https://parts.igem.org/Part:BBa_R0040">R0040</a>) and a ribosome binding site (<a href="https://parts.igem.org/Part:BBa_B0034">B0034</a>). | + | <html><p><i>AmdA</i> coding sequence (<a href="https://parts.igem.org/Part:BBa_K902035">K902035</a>) downstream of a strong TetR repressible promoter (<a href="https://parts.igem.org/Part:BBa_R0040">R0040</a>) and a ribosome binding site (<a href="https://parts.igem.org/Part:BBa_B0034">B0034</a>). AmdA is an amidase enzyme from <i>Rhodococcus erythropolis</i> that can remove amine and amide groups from carbon rings. It can potentially replace the function of the genes responsible for the lower half of the carbazole degradation pathway by cleaving the second C-N bond after the carA enzyme has converted carbazole into 2'-aminobiphenyl-2,3-diol.</p> |
| − | </html>[[Image:amidase pathway kilbane calgary12.jpg|center]] | + | </html>[[Image:amidase pathway kilbane calgary12.jpg|center]]<html> |
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| + | <p>It can also remove the nitrogen from amides and replace it with a hydroxyl group, creating a carboxylic acid. This part's function was characterized using benzamide as a model compound as detailed in the experience section of this page.</p> | ||
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Revision as of 21:19, 26 October 2012
Constitutive AmdA Generator
AmdA coding sequence (K902035) downstream of a strong TetR repressible promoter (R0040) and a ribosome binding site (B0034). AmdA is an amidase enzyme from Rhodococcus erythropolis that can remove amine and amide groups from carbon rings. It can potentially replace the function of the genes responsible for the lower half of the carbazole degradation pathway by cleaving the second C-N bond after the carA enzyme has converted carbazole into 2'-aminobiphenyl-2,3-diol.
It can also remove the nitrogen from amides and replace it with a hydroxyl group, creating a carboxylic acid. This part's function was characterized using benzamide as a model compound as detailed in the experience section of this page.
Sequence and Features