Difference between revisions of "Part:BBa K861074:Experience"
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<p align="center"><img src="https://static.igem.org/mediawiki/igem.org/f/ff/Cellulose_Fig_1.jpg" alt="" name="CelluloseFig1" width="520" height="1247" align="middle" id="CelluloseFig1" /></p> | <p align="center"><img src="https://static.igem.org/mediawiki/igem.org/f/ff/Cellulose_Fig_1.jpg" alt="" name="CelluloseFig1" width="520" height="1247" align="middle" id="CelluloseFig1" /></p> | ||
| − | <p>All these results indicated that AdrA can increase the ability of cellulose synthesis</p> | + | <p>All these results indicated that AdrA can increase the ability of cellulose synthesis.Actually, BcsB can be activated by c-di-GMP. But till the deadline, we have not successfully assembled Pcar and bcsB. In the following work, we are going to assemble a plasmid including all of the seven genes. Maybe in this way, cellulose production will increase greatly.</p> |
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===User Reviews=== | ===User Reviews=== | ||
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Latest revision as of 16:52, 26 October 2012
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K861074
In our experiment, cells that expressed protein which was nothing to do with cellulose synthesis was used as a control.We transformed the seven genes involved in cellulose synthesis into E.coli and meaaured cellulose production. In the step of measuring total cellulose production, exceed cellulase was appended and incubated at 50 ℃ for 1 hour, and results show that the overexpression of adrA can help increase the ability of cellulose synthesis.
And also we co-overexpress adrA with bcsA. Cellulose output in both supernatant and deposit were show in the following figure. The cellulose production in adrA+bcsA is more than 0.6 mg/mL, which is almost two times higher than that of control.
All these results indicated that AdrA can increase the ability of cellulose synthesis.Actually, BcsB can be activated by c-di-GMP. But till the deadline, we have not successfully assembled Pcar and bcsB. In the following work, we are going to assemble a plasmid including all of the seven genes. Maybe in this way, cellulose production will increase greatly.
User Reviews
UNIQ02e64b760b9421b1-partinfo-00000001-QINU UNIQ02e64b760b9421b1-partinfo-00000002-QINU