Difference between revisions of "Part:BBa K861074:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K861074=== | ===Applications of BBa_K861074=== | ||
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| + | <p align="justify">In our experiment, cells that expressed protein which was nothing to do with cellulose synthesis was used as a control.We transformed the seven genes involved in cellulose synthesis into <em>E.coli</em> and meaaured cellulose production. In the step of measuring total cellulose production, exceed cellulase was appended and incubated at 50 ℃ for 1 hour, and results show that the overexpression of adrA can help increase the ability of cellulose synthesis.</p> | ||
| + | <p align="center"><img src="https://static.igem.org/mediawiki/2012/a/a7/CELLULOSE_PRODUCTION.tif" alt="" name="CelluloseFig1" width="520" height="412" align="middle" id="CelluloseFig1" /></p> | ||
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| + | <p align="justify">And also we co-overexpress adrA with bcsA. Cellulose output in both supernatant and deposit were show in the following figure. The cellulose production in <em>adrA</em>+<em>bcsA</em> is more than 0.6 mg/mL, which is almost two times higher than that of control.</p> | ||
| + | <p align="center"><img src="https://static.igem.org/mediawiki/igem.org/f/ff/Cellulose_Fig_1.jpg" alt="" name="CelluloseFig1" width="520" height="1247" align="middle" id="CelluloseFig1" /></p> | ||
| + | <p>All these results indicated that AdrA can increase the ability of cellulose synthesis</p> | ||
| + | </html> | ||
===User Reviews=== | ===User Reviews=== | ||
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Revision as of 16:50, 26 October 2012
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K861074
In our experiment, cells that expressed protein which was nothing to do with cellulose synthesis was used as a control.We transformed the seven genes involved in cellulose synthesis into E.coli and meaaured cellulose production. In the step of measuring total cellulose production, exceed cellulase was appended and incubated at 50 ℃ for 1 hour, and results show that the overexpression of adrA can help increase the ability of cellulose synthesis.
And also we co-overexpress adrA with bcsA. Cellulose output in both supernatant and deposit were show in the following figure. The cellulose production in adrA+bcsA is more than 0.6 mg/mL, which is almost two times higher than that of control.
All these results indicated that AdrA can increase the ability of cellulose synthesis
User Reviews
UNIQa158db830ae275ad-partinfo-00000001-QINU UNIQa158db830ae275ad-partinfo-00000002-QINU