Difference between revisions of "Part:BBa K917005"
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This is the gene (''nfsI'') from ''Enterobacter cloacae'' which encodes nitroreductaseunder the control of the lac promoter. This is an enzyme which reduces nitrogen containing compounds(Nicklin & Bruce, 1998). Nitroreductases were implemented in convering nitro drugs such as metronidazole into their active form which is essential part of their toxicity (Nillius, Muller, & Muller, 2011).Expression of this gene in the presence of  metronidazole leads to growth inhibition (see Experience page). This gene could possibly could be counter selectable marker.
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This is the gene (''nfsI'') from ''Enterobacter cloacae'' which encodes nitroreductaseunder the control of the lac promoter. This is an enzyme which reduces nitrogen containing compounds(Nicklin & Bruce, 1998). Nitroreductases were implemented in convering nitro drugs such as metronidazole into their active form which is essential part of their toxicity (Nillius, Muller, & Muller, 2011).Expression of this gene in the presence of  metronidazole leads to growth inhibition (see Experience page). This gene can be used as a counter selectable marker.
  
 
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Latest revision as of 14:42, 25 October 2012

lac promoter plus nitroreductase (nsfI) gene from Enterobacter cloacae


This is the gene (nfsI) from Enterobacter cloacae which encodes nitroreductaseunder the control of the lac promoter. This is an enzyme which reduces nitrogen containing compounds(Nicklin & Bruce, 1998). Nitroreductases were implemented in convering nitro drugs such as metronidazole into their active form which is essential part of their toxicity (Nillius, Muller, & Muller, 2011).Expression of this gene in the presence of metronidazole leads to growth inhibition (see Experience page). This gene can be used as a counter selectable marker.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1189
  • 1000
    COMPATIBLE WITH RFC[1000]