Difference between revisions of "Part:BBa K917005"

Latest revision as of 14:42, 25 October 2012

lac promoter plus nitroreductase (nsfI) gene from Enterobacter cloacae

This is the gene (nfsI) from Enterobacter cloacae which encodes nitroreductaseunder the control of the lac promoter. This is an enzyme which reduces nitrogen containing compounds(Nicklin & Bruce, 1998). Nitroreductases were implemented in convering nitro drugs such as metronidazole into their active form which is essential part of their toxicity (Nillius, Muller, & Muller, 2011).Expression of this gene in the presence of metronidazole leads to growth inhibition (see Experience page). This gene can be used as a counter selectable marker.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1189
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K917005 short</partinfo>
-This is the gene (nfsI) from Enterobacter cloacae which encodes nitroreductase under the control of the lac promoter.Expression of this gene in the presence of metronidazole leads to growth inhibition. This gene could possibly could be counter selectable marker.
+This is the gene (''nfsI'') from ''Enterobacter cloacae'' which encodes nitroreductaseunder the control of the lac promoter. This is an enzyme which reduces nitrogen containing compounds(Nicklin & Bruce, 1998). Nitroreductases were implemented in convering nitro drugs such as metronidazole into their active form which is essential part of their toxicity (Nillius, Muller, & Muller, 2011).Expression of this gene in the presence of  metronidazole leads to growth inhibition (see Experience page). This gene can be used as a counter selectable marker.
 <!-- Add more about the biology of this part here