Difference between revisions of "Part:BBa K801077:Design"

Revision as of 09:47, 23 October 2012

Caffeine Synthesis Pathway pTEF2-XMT1-tADH1-pTEF1-MXMT1-tADH1-pTEF2-DXMT1-tADH1

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 3613
Illegal BglII site found at 5829
Illegal BglII site found at 5925
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 138
Illegal NgoMIV site found at 4317
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2385

Composite part of BBa_K801010 BBa_K801070 BBa_K801012 BBa_K319003 BBa_K801071 BBa_K801012 BBa_K801010 BBa_K801072 BBa_K801012

Keywords: caffeine, TEF1,

Abbreviations:

Design Notes

Related BioBrick:

Quality control measures:

Backbone:

Backbone name: pSB1C3'

Protein coding:

Enzymatic activity:

Cytotoxicity:
This Biobrick includes all enzymes of the pathway which leads to caffeine. We investigated the toxicity of caffeine and found out, that the growth of yeast strains was inhibited at concentrations of 10 mM. Caffeine with a concentration of 100 mM even showed a lethal effect on yeast cells. Lower concentrations of caffeine (range of micro molar) did not show any growth inhibition or effect.

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:

Organism:

References

Literature references:

Database references:

@@ Line 18: / Line 18: @@
 '''Related BioBrick:'''
-<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
+<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_K801076: Name_of_part] -->
-<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
+<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_K801074 BBa_K801075: Name_of_part]
 '''Cloning details:'''<br>