Difference between revisions of "Part:BBa K801076"
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This BioBrick is a generator for the first two enzymes envolved in caffeine biosynthesis: methylxanthosine N-methyltransferase 1 and 7- methylxanthine N-methyltransferase 1. It is made up of the two single generators for [https://parts.igem.org/Part:BBa_K801073 CaXMT1] and [https://parts.igem.org/Part:BBa_K801074 CaMXMT1], which means that CaXMT1 is regulated by the Tef2 promoter and CaMXMT1 is regulated by the Tef1 promoter. In both cases, the Adh1 terminator was used. | This BioBrick is a generator for the first two enzymes envolved in caffeine biosynthesis: methylxanthosine N-methyltransferase 1 and 7- methylxanthine N-methyltransferase 1. It is made up of the two single generators for [https://parts.igem.org/Part:BBa_K801073 CaXMT1] and [https://parts.igem.org/Part:BBa_K801074 CaMXMT1], which means that CaXMT1 is regulated by the Tef2 promoter and CaMXMT1 is regulated by the Tef1 promoter. In both cases, the Adh1 terminator was used. | ||
| + | =Background and principles= | ||
| + | =References= | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 19:12, 20 October 2012
Yeast expression cassette for CaXMT1 and CaMXMT1
Yeast Expression Cassette for the enzymes 1 and 2 of the caffeine biosynthesis pathway
Expression of CaXMT1 is controlled by the yeast TEF2 promoter and the yeast ADH1 terminator.
Expression of CaMXMT1 is controlled by the yeast TEF1 promoter and the yeast ADH1 terminator.
This BioBrick is a generator for the first two enzymes envolved in caffeine biosynthesis: methylxanthosine N-methyltransferase 1 and 7- methylxanthine N-methyltransferase 1. It is made up of the two single generators for CaXMT1 and CaMXMT1, which means that CaXMT1 is regulated by the Tef2 promoter and CaMXMT1 is regulated by the Tef1 promoter. In both cases, the Adh1 terminator was used.
Background and principles
References
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3613
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 138
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2385