This part, which is a fusion protein, serves as <b>an ultra sensitive photoreceptor</b> (which can sense light as weak as moonlight) and will induce a <b>light-dependent repression</b> of genes under specific promoters like [https://parts.igem.org/wiki/index.php?title=Part:BBa_K819002 RecA408 promoter] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K819017 SulA408 promoter].
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For the photosensor domain of this fusion protein, we chose VVD protein, the smallest photosensor protein known, which forms a rapidly exchanging dimer when excited by blue light. For the physiological function domain, we chose LexA protein, a bacteria transcription inhibitor, whose DNA binding domain rigorously requires help of the dimerization domain for its DNA binding activity. We fused these two separate domains together to form a fusion protein, which we termed our "Luminesensor". Both DNA binding domain and dimerization domain of this luminesensor was mutated at specific sites for better bio-orthogonality and higher on-off ratio.(to see the <b>design</b> page for details, please click [https://parts.igem.org/Part:BBa_K819005:Designing here])
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We then characterized this luminesensor in different ways and all the results showed that this luminesensor could response to light quite well. (to see the <b>characterization</b> page for details, please click [https://parts.igem.org/Part:BBa_K819005:Characterization here])
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To learn more details about this luminesensor and the related project by Peking 2012 iGEM team, please visit our [http://2012.igem.org/Team:Peking wiki]
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Revision as of 04:43, 20 October 2012
Constitutive LuxBrick Generator
Sequence and Features
Assembly Compatibility:
10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 3014
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2012 Illegal XhoI site found at 2842
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 4401 Illegal BsaI.rc site found at 1410 Illegal SapI.rc site found at 4726