Difference between revisions of "Part:BBa K801080:Design"

Latest revision as of 10:52, 15 October 2012

Prepro-Thaumatin, yeast codon optimized

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Synthesized by IDT. The part is codon optimized for yeast. 3 forbidden restriction sites have been removed.

Please note, that the pre- and pro-part are split off, thus there is no possibility to add streptags or form other recombinant proteins.

Based on: http://www.ncbi.nlm.nih.gov/nuccore/121945717

Keywords: preprothaumatin, yeast, sweetner

Abbreviations:

Design Notes

Related BioBrick: -

Cloning details:

Designed in RFC10

Quality control measures:

Part was totally sequenced

Backbone:

Backbone name: pSB1C3
Resistance: Cp
Copynumber: high

Protein coding:

Protein: Preprothaumatin [Nucleotide 1 to 708]
The protein encoded is posttranslationally modified: Aa 1-22 and 229-236 are split off

Enzymatic activity: -

Cytotoxicity:
This Biobrick leads to the production of thaumatin. We investigated the toxicity of thaumatin and incubated yeast cells (strain INVSc) with 45 mM of thaumatin. We found, that thaumatin does not show any negative effect on the growth rate of yeast cells.

Safety notes:

Intellectual property: Information on patent situation: Preprothaumatin derived from Thaumatococcus daniellii

Corresponding part author/authors:

Source

Source:

Synthesized by idt

Organism:

Genesequence derived from Thaumatococcus daniellii
Codonoptimized for S. cerevisiae
Designed for the following Chassis: S. cerevisiae
Functionality in other chassis: Should work in other chassis as well (although optimized for S. cerevisiae)

References

Literature references:

[http://www.ncbi.nlm.nih.gov/pubmed/6327079 Pubmed: Edens L, Bom I, Ledeboer AM, Maat J, Toonen MY, Visser C, Verrips CT., 1984: Synthesis and processing of the plant protein thaumatin in yeast]
[http://www.ncbi.nlm.nih.gov/pubmed/19489607 Pubmed: Ide N, Sato E, Ohta K, Masuda T, Kitabatake N., 2009: Interactions of the sweet-tasting proteins thaumatin and lysozyme with the human sweet-taste receptor]
[http://www.ncbi.nlm.nih.gov/pubmed/17897626 Pubmed: Ide N, Masuda T, Kitabatake N., 2007: Effects of pre- and pro-sequence of thaumatin on the secretion by Pichia pastoris]

Database references:

[http://www.ncbi.nlm.nih.gov/nuccore/121945717 GenBank AB265690.1: Thaumatococcus daniellii mRNA for preprothaumatin I]
[http://http://www.ebi.ac.uk/interpro/IEntry?ac=IPR017949&query=thaumatin Interpro: Thaumatin, conserved site (IPR017949)]
[http://www.uniprot.org/uniprot/A1IIJ1 Uniprot: Preprothaumatin I]

@@ Line 16: / Line 16: @@
 <br>'''Keywords:'''
 <!--These keywords are necessary to find your part using a fulltext sarch.-->
-<!--keyword_1, keyword_2, keyword_3, keyword_4, keyword_5-->
+preprothaumatin, yeast, sweetner
 <br>'''Abbreviations:'''
@@ Line 82: / Line 82: @@
 '''Literature references:'''<br>
-<!--*[http://www.ncbi.nlm.nih.gov/pubmed/?PMID? '''Pubmed:''' ?Author(s)?, ?year?: ?title?]-->
+*[http://www.ncbi.nlm.nih.gov/pubmed/6327079 '''Pubmed:''' Edens L, Bom I, Ledeboer AM, Maat J, Toonen MY, Visser C, Verrips CT., 1984: Synthesis and processing of the plant protein thaumatin in yeast]
+*[http://www.ncbi.nlm.nih.gov/pubmed/19489607 '''Pubmed:''' Ide N, Sato E, Ohta K, Masuda T, Kitabatake N., 2009: Interactions of the sweet-tasting proteins thaumatin and lysozyme with the human sweet-taste receptor]
+*[http://www.ncbi.nlm.nih.gov/pubmed/17897626 '''Pubmed:''' Ide N, Masuda T, Kitabatake N., 2007: Effects of pre- and pro-sequence of thaumatin on the secretion by Pichia pastoris]
 '''Database references:'''<br>