Difference between revisions of "Part:BBa K801000:Design"

Latest revision as of 08:24, 15 October 2012

pTUM100 yeast shuttle vector based on pYES2

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal prefix found at 36
Illegal suffix found at 114
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 36
Illegal NheI site found at 3379
Illegal SpeI site found at 115
Illegal PstI site found at 129
Illegal NotI site found at 42
Illegal NotI site found at 122
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 36
Illegal XhoI site found at 63
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 36
Illegal suffix found at 115
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 36
Illegal suffix found at 105
Illegal XbaI site found at 51
Illegal NgoMIV site found at 69
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 79
Illegal BsaI.rc site found at 1536
Illegal BsaI.rc site found at 2482
Illegal SapI site found at 453
Illegal SapI site found at 2634

Keywords:

yeast shuttle vector, yeast, yeast vector, pTUM, pYES2

Abbreviations:

Design Notes

Related BioBrick:

Other versions:

Quality control measures:

Part was totally sequenced

Backbone:

Protein coding:

Enzymatic activity:

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:
Commercial system: pYES2 , Invitrogen

Organism:

References

Literature references:

http://www.ncbi.nlm.nih.gov/pubmed/6392852 West et al., 1984 West, R. W. J., Yocum, R. R., and Ptashne, M. (1984). Saccharomyces cerevisiae GAL1-GAL10 Divergent Promoter Region: Location and Function of the Upstream Activator Sequence UASG. Mol. Cell. Biol.4, 2467-2478.
http://www.ncbi.nlm.nih.gov/pubmed/3886158 Giniger et al., 1985 Giniger, E., Barnum, S. M., and Ptashne, M. (1985). Specific DNA Binding of GAL4, a Positive Regulatory Protein of Yeast. Cell 40, 767-774.

Database references:

@@ Line 27: / Line 27: @@
 '''Cloning details:'''<br>
-<!--*Designed in RFC10/RFC23/RFC25/RFC25_N-part-->
+*Designed in RFC25
 <!--*Mutation C889G to delete XbaI restriction site-->
 <!--*Truncation upstream/downstream compared to template, ?explanation?-->
@@ Line 34: / Line 34: @@
 <!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
 <!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
-<!--*Part was partly sequenced/Part was totally sequenced-->
+*Part was totally sequenced
 '''Backbone:'''<br>
@@ Line 67: / Line 67: @@
 '''Source:'''<br>
-<!--*Commercial system: plasmid name, system name, company name-->
+Commercial system: pYES2 , Invitrogen
 <!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?-->
 <!--*Preexisting BioBrick ?Bba_number?-->
@@ Line 83: / Line 83: @@
 <!--Thank you very much for using BBF RFC 82 to describe the present BioBrick. If you have any remarks or recommendations concerning this RFC we would highly appreciate to get your feedback on: []-->
 ===References===
@@ Line 89: / Line 88: @@
 '''Literature references:'''<br>
-<!--*[http://www.ncbi.nlm.nih.gov/pubmed/?PMID? '''Pubmed:''' ?Author(s)?, ?year?: ?title?]-->
+*[[http://www.ncbi.nlm.nih.gov/pubmed/6392852 West et al., 1984]] West, R. W. J., Yocum, R. R., and Ptashne, M. (1984). Saccharomyces cerevisiae GAL1-GAL10 Divergent Promoter Region: Location and Function of the Upstream Activator Sequence UASG. ''Mol. Cell. Biol.''4, 2467-2478.
+*[[http://www.ncbi.nlm.nih.gov/pubmed/3886158 Giniger et al., 1985]] Giniger, E., Barnum, S. M., and Ptashne, M. (1985). Specific DNA Binding of GAL4, a Positive Regulatory Protein of Yeast. ''Cell'' 40, 767-774.
 '''Database references:'''<br>