Difference between revisions of "Part:BBa K779602"

Latest revision as of 04:20, 11 October 2012

Hef1a-TagBFP-FF5 MammoBlock Device

A composite part with a constitutive Hef1A promoter with a TagBFP (blue fluorescent protein) appended with microRNA binding sites. Created through the gateway cloning protocol.

We transfected 250 ng of Hef1A:TagBFP-FF5 into 100,000 HEK293 cells using Lipofectamine 2000 reagent. Images were taken on a Leica Confocal microscope at 10X. Left shows a brightfield image. Right shows the blue channel. We can observe high transfection efficiency and expression of TagBFP.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 315
Illegal PstI site found at 820
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 315
Illegal PstI site found at 820
Illegal NotI site found at 1285
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 569
Illegal XhoI site found at 968
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 315
Illegal PstI site found at 820
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 315
Illegal PstI site found at 820
Illegal NgoMIV site found at 703
Illegal AgeI site found at 81
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1918

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K779602 short</partinfo>
-to be completetd by MIT iGEM 2012 soon
+A composite part with a constitutive Hef1A promoter with a TagBFP (blue fluorescent protein) appended with microRNA binding sites. Created through the gateway cloning protocol.
+<br>
+<br>
+[[Image:Tagchar.png]]
+<br>
+<i> We transfected 250 ng of Hef1A:TagBFP-FF5 into 100,000 HEK293 cells using Lipofectamine 2000 reagent. Images were taken on a Leica Confocal microscope at 10X. Left shows a brightfield image. Right shows the blue channel. We can observe high transfection efficiency and expression of TagBFP. </i>
 <!-- Add more about the biology of this part here