Difference between revisions of "Part:BBa K779601"

 
 
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<partinfo>BBa_K779601 short</partinfo>
 
<partinfo>BBa_K779601 short</partinfo>
  
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to be completetd by MIT iGEM 2012 soon
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A composite part with a Hef1A constitutive promoter with a mammalian-codon optimized red fluorescent protein. This protein is a significantly engineered reporter protein that, unlikely many fluorescent proteins, is monomeric and therefore ideal for fusion constructs.
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[[Image:MKatechar.png]]
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<i>200,000 cells were transiently transfected with 500 ng of Hef1A:mKate using Lipofectamine 2000. Images were taken on an Evos microscope at 10X. The left image is brightfield. The right is in the Texas-Red channel. Very high transfection efficiency and mKate expression levels can be observed when mKate is driven by the constitutive promoter Hef1A (BBa_K779200).</i>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 04:17, 11 October 2012

Hef1a-mKate MammoBlock Device

A composite part with a Hef1A constitutive promoter with a mammalian-codon optimized red fluorescent protein. This protein is a significantly engineered reporter protein that, unlikely many fluorescent proteins, is monomeric and therefore ideal for fusion constructs.

MKatechar.png
200,000 cells were transiently transfected with 500 ng of Hef1A:mKate using Lipofectamine 2000. Images were taken on an Evos microscope at 10X. The left image is brightfield. The right is in the Texas-Red channel. Very high transfection efficiency and mKate expression levels can be observed when mKate is driven by the constitutive promoter Hef1A (BBa_K779200).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 315
    Illegal PstI site found at 820
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 315
    Illegal PstI site found at 820
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 569
    Illegal XhoI site found at 968
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 315
    Illegal PstI site found at 820
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 315
    Illegal PstI site found at 820
    Illegal NgoMIV site found at 703
    Illegal AgeI site found at 81
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1837
    Illegal SapI.rc site found at 1219