Difference between revisions of "Part:BBa K732001:Experience"

Revision as of 04:01, 10 October 2012

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Applications of BBa_K732001

One application of this component is detection by pregnancy test.

When heterogeneous mixture of E. coli expressing BBa_K732001 and BBa_K732002 was lysed by either sonication or T7 infection, re-folded using a literature-based protocol, and applied to NewChoice pregnancy tests (purchased from Dollar Tree), positive results were visible.

Note that (a) there was some coloration around the positive band, and (b) the pregnancy tests were left overnight for bands to become visible. We hypothesize that the delayed read-time is necessary because the folding solution interferes with band visibility.

User Reviews

UNIQc198637862d5b2b8-partinfo-00000000-QINU UNIQc198637862d5b2b8-partinfo-00000001-QINU

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 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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 ===Applications of BBa_K732001===
+One application of this component is detection by pregnancy test.
+When heterogeneous mixture of ''E. coli'' expressing BBa_K732001 and BBa_K732002 was lysed by either sonication or T7 infection, re-folded using a literature-based protocol, and applied to NewChoice pregnancy tests (purchased from Dollar Tree), positive results were visible.
+Note that (a) there was some coloration around the positive band, and (b) the pregnancy tests were left overnight for bands to become visible. We hypothesize that the delayed read-time is necessary because the folding solution interferes with band visibility.
+[[Image:Virginia 2012 Oct_2_Preg_Test_Results.jpg]]
 ===User Reviews===