Difference between revisions of "Part:BBa K783067"
 
(5 intermediate revisions by the same user not shown)
Line 3: Line 3:
  
 
This is an inverter with pBad driving tetR with GFP as a reporter. pTetR has RFP has a reporter. We used a weaker RBS (B0032) to control the tetR expression and it helped the inverter function properly.
 
This is an inverter with pBad driving tetR with GFP as a reporter. pTetR has RFP has a reporter. We used a weaker RBS (B0032) to control the tetR expression and it helped the inverter function properly.
 +
 +
We used flow cytometry to measure the function of our inverter. As arabinose concentration increases, tetR and GFP also increases. As the tetR amount increases, the RFP decreases as tetR represses the pTetR promoter controlling RFP.
 +
 +
[[image:inverter.png|550px|center]]
 +
 +
This result showed an improvement over an inverter we built with the B0034 RBS controlling the tetR gene (shown below). The gradual change in GFP and RFP seen above shows a finer control over the circuit than the dramatic drop off of RFP below. The GFP below is also very erratic.
 +
 +
[[image:inverterbad.png|550px|center]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 01:52, 10 October 2012

pBad-pTetR inverter

This is an inverter with pBad driving tetR with GFP as a reporter. pTetR has RFP has a reporter. We used a weaker RBS (B0032) to control the tetR expression and it helped the inverter function properly.

We used flow cytometry to measure the function of our inverter. As arabinose concentration increases, tetR and GFP also increases. As the tetR amount increases, the RFP decreases as tetR represses the pTetR promoter controlling RFP.

Inverter.png

This result showed an improvement over an inverter we built with the B0034 RBS controlling the tetR gene (shown below). The gradual change in GFP and RFP seen above shows a finer control over the circuit than the dramatic drop off of RFP below. The GFP below is also very erratic.

Inverterbad.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1247
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1187
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 3490
    Illegal AgeI site found at 3602
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2634
    Illegal SapI site found at 961