Difference between revisions of "Part:BBa K935002:Experience"

(→‎Applications of BBa_K935002)
 
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how you used this part and how it worked out.
 
how you used this part and how it worked out.
  
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[[Image:BBa_K935002_Response_to_Arsenate.jpg]]
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'''Response of BBa_935002 in E.coli.'''
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A dilution series of sodium arsenate was created in 100 uL of LB broth in 96-well tissue culture plates.  Replicate 100ul of cells were plated at a density of 2.5*10^8 cells per mL  and incubated for 12 hrs at 37C.  Epifluoresence (Ex518/Em607) was measured on a Molecular Devices FilterMax F3 reader (PMT 2 sec) and divided by the cell number determined by an A600 scan of the same wells to derive the flourescence per cell (which was multiplied by 10^4 for charting convenience). Error bars represent standard deviation of the sample mean.
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===User Reviews===
 
===User Reviews===

Latest revision as of 15:13, 6 October 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

BBa K935002 Response to Arsenate.jpg

User Reviews

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