Difference between revisions of "Part:BBa K945000"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K945000 short</partinfo>
 
<partinfo>BBa_K945000 short</partinfo>
  
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This sequence contains the strong inducible promoter AOX1, useful for controlled gene expression which is actively enhanced in the presence of methanol.
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This sequence contains the strong inducible transcription promoter AOX1, useful for controlled gene expression which is actively enhanced in <i>Pichia pastoris</i> in the presence of methanol.
  
 
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<partinfo>BBa_K945000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K945000 SequenceAndFeatures</partinfo>
  
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===Promoter Strength===
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Tec-Monterrey Ekam is working on '''quantitatively''' characterizing the functionality of this part through the use of <partinfo>BBa_K945004</partinfo> (GFP) as a reporter, which will be finalized shortly.
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 10:47, 6 October 2012

AOX1 yeast promoter

This sequence contains the strong inducible transcription promoter AOX1, useful for controlled gene expression which is actively enhanced in Pichia pastoris in the presence of methanol.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1016
    Illegal PstI site found at 47
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 47
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 53
    Illegal BglII site found at 75
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1016
    Illegal PstI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1016
    Illegal PstI site found at 47
    Illegal NgoMIV site found at 34
  • 1000
    COMPATIBLE WITH RFC[1000]

Promoter Strength

Tec-Monterrey Ekam is working on quantitatively characterizing the functionality of this part through the use of BBa_K945004 (GFP) as a reporter, which will be finalized shortly.