Difference between revisions of "Part:BBa K769003:Design"

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===Design Notes===
 
===Design Notes===
−
[http://2012.igem.org/Team:Tokyo-NoKoGen/Project/rhodopsin]
 
  
 
https://static.igem.org/mediawiki/2012/thumb/c/cb/Rhodopsin2.jpg/800px-Rhodopsin2.jpg
 
https://static.igem.org/mediawiki/2012/thumb/c/cb/Rhodopsin2.jpg/800px-Rhodopsin2.jpg
 +
 +
https://static.igem.org/mediawiki/2012/thumb/d/d6/Rhodopsin3.jpg/800px-Rhodopsin3.jpg
  
 
===Source===
 
===Source===
  
−
Halobacteria
+
 
 +
SRII and HtrII : <i>Natronomonas pharaonis</i>
 +
<br>
 +
EnvZ : <i>E coli</i>
  
 
===References===
 
===References===

Latest revision as of 07:50, 6 October 2012

Pconst.(High)-RBS-NpSRII-9a.a.linker_NpHtrII-EnvZ-Double terminator-PompR-RBS-GFP-Double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2626
    Illegal XhoI site found at 2527
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 134
    Illegal NgoMIV site found at 392
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1088
    Illegal SapI site found at 862


Design Notes

800px-Rhodopsin2.jpg

800px-Rhodopsin3.jpg

Source

SRII and HtrII : Natronomonas pharaonis
EnvZ : E coli

References