Difference between revisions of "Part:BBa K855000"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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BBa_K855000 is inserted into commercial expression vector pET-21(a) whose transcription is under the control of T7 promoter and a modified lac operon system. The recombinant vector is transformed into BL21 expression host cell. After the culture reaches an OD of 0.6, the protein expression is induced using 0.5mM IPTG. | BBa_K855000 is inserted into commercial expression vector pET-21(a) whose transcription is under the control of T7 promoter and a modified lac operon system. The recombinant vector is transformed into BL21 expression host cell. After the culture reaches an OD of 0.6, the protein expression is induced using 0.5mM IPTG. | ||
Revision as of 07:21, 6 October 2012
pvdQ-B0015
This part consists of the gene pvdQ isolated from Pseudomonas areuginosa. pvdQ is an acylase that is capable of degrading long-chain acyl homoserine lactone (AHL) molecules with 6 to 12 carbons.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1489
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1489
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1196
Illegal XhoI site found at 1594 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1489
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1489
Illegal NgoMIV site found at 31
Illegal NgoMIV site found at 770
Illegal NgoMIV site found at 1172
Illegal NgoMIV site found at 1329
Illegal NgoMIV site found at 1556 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1926