Difference between revisions of "Part:BBa K778004"
YutaOtsuka (Talk | contribs) |
YutaOtsuka (Talk | contribs) |
||
| (One intermediate revision by the same user not shown) | |||
| Line 6: | Line 6: | ||
FhlA is the trnascriptional activator of hycAp [[Part:BBa K778000]] . | FhlA is the trnascriptional activator of hycAp [[Part:BBa K778000]] . | ||
E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)). | E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)). | ||
| − | |||
| + | [[Image:UT Tokyo H2 1001+eg.png]] | ||
| + | <!--[[Image:UT Tokyo H2 1001.png]]--> | ||
| + | |||
| + | (The full construct in this experiment is [[Part:BBa K778007]] and | ||
| + | the modified fhlA(E363G) part is [[Part:BBa K778002]].) | ||
| + | |||
| + | Detailed Information : Our wiki(UT-Tokyo 2012)[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli] | ||
| + | The results shown on the wiki are different from the latest result on this parts.igem page. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 06:17, 6 October 2012
RBS-fhlA-d.term
FhlA generator without promoter (RBS-fhlA-d.term)
FhlA is the trnascriptional activator of hycAp Part:BBa K778000 . E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)).
(The full construct in this experiment is Part:BBa K778007 and the modified fhlA(E363G) part is Part:BBa K778002.)
Detailed Information : Our wiki(UT-Tokyo 2012)[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli] The results shown on the wiki are different from the latest result on this parts.igem page.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2115
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1394
- 1000COMPATIBLE WITH RFC[1000]