Difference between revisions of "Part:BBa K778004"

Latest revision as of 06:17, 6 October 2012

RBS-fhlA-d.term

FhlA generator without promoter (RBS-fhlA-d.term)

FhlA is the trnascriptional activator of hycAp Part:BBa K778000 . E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)).

(The full construct in this experiment is Part:BBa K778007 and the modified fhlA(E363G) part is Part:BBa K778002.)

Detailed Information : Our wiki(UT-Tokyo 2012)[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli] The results shown on the wiki are different from the latest result on this parts.igem page.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2115
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1394
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 FhlA generator without promoter (RBS-fhlA-d.term)
-FhlA is the trnascriptional activator of hycAp ([[Part:BBa 778000]]).
+FhlA is the trnascriptional activator of hycAp [[Part:BBa K778000]] .
 E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)).
-Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]
+[[Image:UT Tokyo H2 1001+eg.png]]
+<!--[[Image:UT Tokyo H2 1001.png]]-->
+(The full construct in this experiment is [[Part:BBa K778007]] and
+the modified fhlA(E363G) part is [[Part:BBa K778002]].)
+Detailed Information : Our wiki(UT-Tokyo 2012)[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]
+The results shown on the wiki are different from the latest result on this parts.igem page.
 <!-- Add more about the biology of this part here