Difference between revisions of "Part:BBa K838001:Design"

 
(→‎Source)
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K838001 short</partinfo>
 
<partinfo>BBa_K838001 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
Has 2 SpeI and 1 EcoRI site init
+
This part contains two illegal SpeI sites and one illegal EcoRI site.
−
 
+
We planned on performing site directed mutagenesis but unfortunately the kit we needed didn't arrive on time due to issues and the attempts we had weren't successful yet.
  
 +
The part can still be cut out with XbaI and PstI.
  
 
===Source===
 
===Source===
  
−
Nicolas Gobet
+
Nicolas Gobet, former EPF Lausanne iGEM member.
  
 
===References===
 
===References===

Latest revision as of 18:02, 4 October 2012

LovTAP readout


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 424
    Illegal SpeI site found at 295
    Illegal SpeI site found at 303
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 424
    Illegal SpeI site found at 295
    Illegal SpeI site found at 303
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 424
    Illegal BglII site found at 381
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 424
    Illegal SpeI site found at 295
    Illegal SpeI site found at 303
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 424
    Illegal SpeI site found at 295
    Illegal SpeI site found at 303
    Illegal NgoMIV site found at 7
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part contains two illegal SpeI sites and one illegal EcoRI site. We planned on performing site directed mutagenesis but unfortunately the kit we needed didn't arrive on time due to issues and the attempts we had weren't successful yet.

The part can still be cut out with XbaI and PstI.

Source

Nicolas Gobet, former EPF Lausanne iGEM member.

References