Difference between revisions of "Part:BBa K864446"
 
Line 14: Line 14:
 
NNNNNNNNNNNNNNNNNNNN<b>GCTAGCATAATACCTAGGAC
 
NNNNNNNNNNNNNNNNNNNN<b>GCTAGCATAATACCTAGGAC
  
−
Tm1 = 52,9°C  
+
Tm = 52,9°C  
−
 
+
−
Tm2 > 72°C
+
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 18:00, 4 October 2012

Spot42 randomized reverse primer


This part is a component of the Uppsala University iGEM 2012 modular small RNA screening system.

This primer is used to create a random sRNA library, together with BBa_K864445, by randomizing the mRNA-binding region of the spot42 small RNA (BBa_K864444). This library can be used to screen for synthetic silencing sRNA:s.

Note

Due to technical limitations, the correct sequence can not be entered into the registry. The entered sequence should be preceded by 20 N:s, to denote the randomized region, and thus read:

NNNNNNNNNNNNNNNNNNNNGCTAGCATAATACCTAGGAC

Tm = 52,9°C

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]