Difference between revisions of "Part:BBa K838002:Design"

 
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===Design Notes===
 
===Design Notes===
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PstI site inside
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The melanopsin gene contains three illegal PstI sites. We noticed that many genes meant to be expressed in mammalian cells contain PstI sites, which makes the standardising of such genes more challenging, because site directed mutagenesis is required.
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We planned on performing mutagenesis on all our Biobricks, but unfortunately we couldn't get the kit to arrive on time and get the mutagenesis to work. But we might try again.
  
 
===Source===
 
===Source===
  
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Fussenegger
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We obtained the melanopsin gene by asking  [http://www.sciencemag.org/content/332/6037/1565.abstract Prof. Martin Fussenegger] to send us his pcDNA3.1-Melanopsin (pHY42) plasmid he used for his experiments.
  
 
===References===
 
===References===
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A synthetic optogenetic transcription device enhances blood-glucose homeostasis in mice.
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Ye H, Baba M, Peng R-W, Fussensgger M.
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Science, 2011 Vol. 332 no. 6037 pp. 1565-1568 [http://www.sciencemag.org/content/332/6037/1565.abstract URL]

Latest revision as of 17:57, 4 October 2012

Melanopsin


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 282
    Illegal PstI site found at 853
    Illegal PstI site found at 1311
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 282
    Illegal PstI site found at 853
    Illegal PstI site found at 1311
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1369
    Illegal BamHI site found at 45
    Illegal BamHI site found at 541
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 282
    Illegal PstI site found at 853
    Illegal PstI site found at 1311
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 282
    Illegal PstI site found at 853
    Illegal PstI site found at 1311
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 711


Design Notes

The melanopsin gene contains three illegal PstI sites. We noticed that many genes meant to be expressed in mammalian cells contain PstI sites, which makes the standardising of such genes more challenging, because site directed mutagenesis is required.

We planned on performing mutagenesis on all our Biobricks, but unfortunately we couldn't get the kit to arrive on time and get the mutagenesis to work. But we might try again.

Source

We obtained the melanopsin gene by asking [http://www.sciencemag.org/content/332/6037/1565.abstract Prof. Martin Fussenegger] to send us his pcDNA3.1-Melanopsin (pHY42) plasmid he used for his experiments.

References

A synthetic optogenetic transcription device enhances blood-glucose homeostasis in mice.

Ye H, Baba M, Peng R-W, Fussensgger M.

Science, 2011 Vol. 332 no. 6037 pp. 1565-1568 [http://www.sciencemag.org/content/332/6037/1565.abstract URL]