Difference between revisions of "Part:BBa K838002:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | PstI site | + | The melanopsin gene contains three illegal PstI sites. We noticed that many genes meant to be expressed in mammalian cells contain PstI sites, which makes the standardising of such genes more challenging, because site directed mutagenesis is required. |
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| + | We planned on performing mutagenesis on all our Biobricks, but unfortunately we couldn't get the kit to arrive on time and get the mutagenesis to work. But we might try again. | ||
===Source=== | ===Source=== | ||
Revision as of 17:55, 4 October 2012
Melanopsin
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 282
Illegal PstI site found at 853
Illegal PstI site found at 1311 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 282
Illegal PstI site found at 853
Illegal PstI site found at 1311 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1369
Illegal BamHI site found at 45
Illegal BamHI site found at 541 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 282
Illegal PstI site found at 853
Illegal PstI site found at 1311 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 282
Illegal PstI site found at 853
Illegal PstI site found at 1311 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 711
Design Notes
The melanopsin gene contains three illegal PstI sites. We noticed that many genes meant to be expressed in mammalian cells contain PstI sites, which makes the standardising of such genes more challenging, because site directed mutagenesis is required.
We planned on performing mutagenesis on all our Biobricks, but unfortunately we couldn't get the kit to arrive on time and get the mutagenesis to work. But we might try again.
Source
Fussenegger