Difference between revisions of "Part:BBa K900000:Design"
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<partinfo>BBa_K900000 short</partinfo> | <partinfo>BBa_K900000 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | The gene was codon optimized for yeast and internal restriction sites were removed for compatibility with | + | The gene was codon optimized for yeast and internal restriction sites were removed for compatibility with the 2012 Berkeley iGEM team's Golden Gate cloning scheme. |
===Source=== | ===Source=== | ||
| − | Synthesized in house. | + | Synthesized in house. Coding sequence was obtained from an engineered version of mCherry, documented in Subach et al., 2009. Of the multiple versions of PAmCherry described in the paper, we synthesized PAmCherry2. |
===References=== | ===References=== | ||
Subach, F. V. et al. Photoactivatable mCherry for high-resolution two-color fluorescence microscopy. Nature Methods 6, 153–159 (2009). | Subach, F. V. et al. Photoactivatable mCherry for high-resolution two-color fluorescence microscopy. Nature Methods 6, 153–159 (2009). | ||
Latest revision as of 15:00, 4 October 2012
PAmCherry (photoactivatable red fluorescent protein)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 682
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The gene was codon optimized for yeast and internal restriction sites were removed for compatibility with the 2012 Berkeley iGEM team's Golden Gate cloning scheme.
Source
Synthesized in house. Coding sequence was obtained from an engineered version of mCherry, documented in Subach et al., 2009. Of the multiple versions of PAmCherry described in the paper, we synthesized PAmCherry2.
References
Subach, F. V. et al. Photoactivatable mCherry for high-resolution two-color fluorescence microscopy. Nature Methods 6, 153–159 (2009).