Difference between revisions of "Part:BBa K900000:Design"
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<partinfo>BBa_K900000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K900000 SequenceAndFeatures</partinfo>
  
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PAmCHERRY is a photoactivatable red fluorescent protein. PAmCherry fluoresces when exposed to 350–400 nm light while does not fluoresce at other wavelengths. It is used a reporter tag; by expressing in cells or localizing to sub-cellular locations, one can gain spatial information within the cell by track organelles, proteins, and the whole cell itself.  One can visualize the photoactivated PAmCherry with the same filter sets used for other red fluorescent proteins.
 
  
 
===Design Notes===
 
===Design Notes===
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Codon optimization for yeast and internal restriction site removal to be compatible with our Golden Gate cloning scheme.  
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The gene was codon optimized for yeast and internal restriction sites were removed for compatibility with the 2012 Berkeley iGEM team's Golden Gate cloning scheme.
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===Source===
 
===Source===
  
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Synthesized in house. Non-genomic DNA sequence. Mutated version of RFP.
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Synthesized in house. Coding sequence was obtained from an engineered version of mCherry, documented in Subach et al., 2009. Of the multiple versions of PAmCherry described in the paper, we synthesized PAmCherry2.
  
 
===References===
 
===References===
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Subach, F. V. et al. Photoactivatable mCherry for high-resolution two-color fluorescence microscopy. Nature Methods 6, 153–159 (2009).

Latest revision as of 15:00, 4 October 2012

PAmCherry (photoactivatable red fluorescent protein)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 682
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene was codon optimized for yeast and internal restriction sites were removed for compatibility with the 2012 Berkeley iGEM team's Golden Gate cloning scheme.

Source

Synthesized in house. Coding sequence was obtained from an engineered version of mCherry, documented in Subach et al., 2009. Of the multiple versions of PAmCherry described in the paper, we synthesized PAmCherry2.

References

Subach, F. V. et al. Photoactivatable mCherry for high-resolution two-color fluorescence microscopy. Nature Methods 6, 153–159 (2009).