Difference between revisions of "Part:BBa K900001:Design"

 
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<partinfo>BBa_K900001 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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Codon optimization for yeast and internal restriction site removal to be compatible with our Golden Gate cloning scheme.  
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This version of PAGFP contains the A206K mutation, which should disrupt fluorescent protein dimerization even at high concentrations. Restriction sites were removed to be compatible with the 2012 Berkeley iGEM team's Golden Gate cloning scheme.
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===Source===
 
===Source===
  
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Synthesized in house. Non-genomic DNA sequence. Mutated version of RFP.
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Cloned off of Addgene plasmid [http://www.addgene.org/11911/ 11911]. Previously engineered version of GFP from Patterson and Lippincott-Schwartz, 2002.
  
 
===References===
 
===References===
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Patterson, G. H. & Lippincott-Schwartz, J. A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells. Science 297, 1873–1877 (2002).

Latest revision as of 14:54, 4 October 2012

PAGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This version of PAGFP contains the A206K mutation, which should disrupt fluorescent protein dimerization even at high concentrations. Restriction sites were removed to be compatible with the 2012 Berkeley iGEM team's Golden Gate cloning scheme.

Source

Cloned off of Addgene plasmid [http://www.addgene.org/11911/ 11911]. Previously engineered version of GFP from Patterson and Lippincott-Schwartz, 2002.

References

Patterson, G. H. & Lippincott-Schwartz, J. A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells. Science 297, 1873–1877 (2002).