Difference between revisions of "Part:BBa K804010:Experience"

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This part consists of an arabinose promoter, a strong RBS, and the metA coding gene. It is able to complement a metA knockout, and the growth rate appears to be proportional to the amount of arabinose that is added.
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===MetA Knockout Complementation by British Columbia iGEM 2012===
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https://static.igem.org/mediawiki/parts/thumb/d/d3/MetA_complementation_no_fluor.png/800px-MetA_complementation_no_fluor.png
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To generate this graph, we cultured the metA auxotroph transformed with this part in M9 media and added the described amount of arabinose. The OD600 of the cultures were measured every 15 minutes, and was run overnight.
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This part consists of an arabinose promoter, a strong RBS, and the MetA coding gene. It is able to complement a metA knockout, and the growth rate appears to be proportional to the amount of arabinose that is added.  
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https://static.igem.org/mediawiki/parts/thumb/d/d3/MetA_complementation_no_fluor.png/800px-MetA_complementation_no_fluor.png
  
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- [[User:jacobtoth|Jacob Toth]]
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To generate this graph we cultured an E. coli MetA knockout transformed with this part in M9 minimal media with the indicated concentrations of arabinose, and measured the OD600 every 15 minutes.

Latest revision as of 03:59, 4 October 2012

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MetA Knockout Complementation by British Columbia iGEM 2012

This part consists of an arabinose promoter, a strong RBS, and the MetA coding gene. It is able to complement a metA knockout, and the growth rate appears to be proportional to the amount of arabinose that is added. 800px-MetA_complementation_no_fluor.png

To generate this graph we cultured an E. coli MetA knockout transformed with this part in M9 minimal media with the indicated concentrations of arabinose, and measured the OD600 every 15 minutes.