Difference between revisions of "Part:BBa K804008:Experience"

Revision as of 03:42, 4 October 2012

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Applications of BBa_K804008

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UNIQbc03c4e99787b963-partinfo-00000000-QINU UNIQbc03c4e99787b963-partinfo-00000001-QINU

TrpA Knockout Complementation by British Columbia iGEM 2012

This part consists of an arabinose promoter, a strong RBS, and the TrpA coding gene. It is able to complement a metA knockout, and the growth rate appears to be proportional to the amount of arabinose that is added.

To generate this graph we cultured an E. coli TrpA knockout transformed with this part in M9 minimal media with the indicated concentrations of arabinose, and measured the OD600 every 15 minutes. The growth at

- Jacob Toth

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+===TrpA Knockout Complementation by British Columbia iGEM 2012===
+This part consists of an arabinose promoter, a strong RBS, and the TrpA coding gene. It is able to complement a metA knockout, and the growth rate appears to be proportional to the amount of arabinose that is added.
+https://static.igem.org/mediawiki/parts/thumb/0/04/TrpA_Complementation_Test_No_fluor.png/800px-TrpA_Complementation_Test_No_fluor.png
+To generate this graph we cultured an E. coli TrpA knockout transformed with this part in M9 minimal media with the indicated concentrations of arabinose, and measured the OD600 every 15 minutes. The growth at
+- [[User:jacobtoth|Jacob Toth]]