Difference between revisions of "Part:BBa K804010:Experience"

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===MetA Knockout Complementation by British Columbia iGEM 2012===
  
 
This part consists of an arabinose promoter, a strong RBS, and the metA coding gene. It is able to complement a metA knockout, and the growth rate appears to be proportional to the amount of arabinose that is added.  
 
This part consists of an arabinose promoter, a strong RBS, and the metA coding gene. It is able to complement a metA knockout, and the growth rate appears to be proportional to the amount of arabinose that is added.  

Revision as of 03:36, 4 October 2012

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Applications of BBa_K804010

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UNIQbfaf427583e19e0c-partinfo-00000000-QINU UNIQbfaf427583e19e0c-partinfo-00000001-QINU

MetA Knockout Complementation by British Columbia iGEM 2012

This part consists of an arabinose promoter, a strong RBS, and the metA coding gene. It is able to complement a metA knockout, and the growth rate appears to be proportional to the amount of arabinose that is added. 800px-MetA_complementation_no_fluor.png

To generate this graph we cultured the MetA knockout transformed with this part in M9 minimal media with the indicated concentrations of arabinose, and measured the OD600 every 15 minutes.

- Jacob Toth