Difference between revisions of "Part:BBa K804011:Experience"
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| + | ===MetA Knockout Complementation by British Columbia iGEM 2012=== | ||
This part was able to complement a MetA knockout, with growth increasing with increasing arabinose concentration. The following graph also shows the MetA knockout without the complementary gene. | This part was able to complement a MetA knockout, with growth increasing with increasing arabinose concentration. The following graph also shows the MetA knockout without the complementary gene. | ||
https://static.igem.org/mediawiki/parts/thumb/f/ff/MetA_complementation.png/800px-MetA_complementation.png | https://static.igem.org/mediawiki/parts/thumb/f/ff/MetA_complementation.png/800px-MetA_complementation.png | ||
To generate this graph we cultured the MetA knockout transformed with this part in M9 minimal media with the indicated concentrations of arabinose, and measured the OD600 every 15 minutes. | To generate this graph we cultured the MetA knockout transformed with this part in M9 minimal media with the indicated concentrations of arabinose, and measured the OD600 every 15 minutes. | ||
- [[User:jacobtoth|Jacob Toth]] | - [[User:jacobtoth|Jacob Toth]] | ||
Latest revision as of 03:36, 4 October 2012
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K804011
User Reviews
UNIQ7795ccb054aec9e1-partinfo-00000000-QINU UNIQ7795ccb054aec9e1-partinfo-00000001-QINU
MetA Knockout Complementation by British Columbia iGEM 2012
This part was able to complement a MetA knockout, with growth increasing with increasing arabinose concentration. The following graph also shows the MetA knockout without the complementary gene.
To generate this graph we cultured the MetA knockout transformed with this part in M9 minimal media with the indicated concentrations of arabinose, and measured the OD600 every 15 minutes.
- Jacob Toth