Difference between revisions of "Part:BBa K902019:Design"

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===Design Notes===
 
===Design Notes===
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The S7 nuclease was synthesized from IDT however there was a point mutation in the sequence that hindered the activity of the nuclease. Instead of the 'A' there was a 'T'. We did mutagenesis to change the mutation back to its original nucleotide. This brick has the S7 that has undergone mutagenesis and is functional.  
+
The S7 nuclease was synthesized from IDT however there was a point mutation in the sequence that hindered the activity of the nuclease. Instead of the 'A' there was a 'T'. We did mutagenesis to change the mutation back to its original nucleotide. This brick has the S7 that has undergone mutagenesis and is functional.
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GTTTCTTCGA ATTCGCGGCC GCTTCTAGAT GACAGAGTAT CTCCTAAGCG CGGGCATCTG
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TATGGCCATT GTTTCGATCT TATTGATCGG CATGGCGATA TCGAACGTAT CTAAGGGCCA
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−
GTACGCGAAA CGCTTCTTCT TTTTCGCCAC GAGTTGCCTT GTCTTAACAT TAGTTGTTGT
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−
TTCTTCACTG TCGTCCAGTG CGAACGCCTC TCAAACCGAT AACGGTGTAA ATCGCAGCGG
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−
GAGCGAAGAT CCTACCGTGT ATTCCGCGAC CTCAACGAAA AAACTTCATA AGGAGCCCGC
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−
TACGCTCATC A*A*AGCCATTG ACGGAGACAC TGTAAAGCTG ATGTACAAAG GCCAACCGAT
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GACCTTTCGC TTGCTGCTGG TCGACACCCC AGAGACGAAA CATCCGAAAA AGGGCGTCGA
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−
GAAATACGGC CCGGAAGCAA GTGCGTTTAC CAAGAAGATG GTGGAAAATG CGAACAAAAT
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−
TGAAGTCGAA TTTGATAAAG GGCAGCGTAC CGACAAATAT GGTCGTGGAC TGGCCTACAT
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−
TTATGCTGAT GGTAAAATGG TGAATGAAGC ACTGGTGCGG CAGGGTCTGG CCAAAGTGGC
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TTATGTGTAT AAACCGAATA ATACTCACGA ACAGCTGCTG CGTAAATCCG AAGCACAGGC
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AAAAAAAGAA AAACTGAACA TTTGGAGCGA AGATAACGCG GATAGCGGTC AATAATAATA
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ATACTAGTAG CGGCCGCTGC AGGAAGAAAC
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===Source===
 
===Source===
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===References===
 
===References===
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1) NCBI no. 57634611 (S7 nuclease sequence)</br>
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2) Dingwall C, Lomonossoff GP, Laskey RA. High sequence specificity of micrococcal nuclease. Nucleic Acids Res 1981 Jun 25;9(12):2659-2673.

Latest revision as of 03:30, 4 October 2012

S7 micrococcal nuclease


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The S7 nuclease was synthesized from IDT however there was a point mutation in the sequence that hindered the activity of the nuclease. Instead of the 'A' there was a 'T'. We did mutagenesis to change the mutation back to its original nucleotide. This brick has the S7 that has undergone mutagenesis and is functional.

Source

This part was synthesized from IDT and is native to Staphylococcus aureus

References

1) NCBI no. 57634611 (S7 nuclease sequence)</br>

2) Dingwall C, Lomonossoff GP, Laskey RA. High sequence specificity of micrococcal nuclease. Nucleic Acids Res 1981 Jun 25;9(12):2659-2673.