Difference between revisions of "Part:BBa K902019:Design"

 
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<partinfo>BBa_K902019 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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It had to be mutated
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The S7 nuclease was synthesized from IDT however there was a point mutation in the sequence that hindered the activity of the nuclease. Instead of the 'A' there was a 'T'. We did mutagenesis to change the mutation back to its original nucleotide. This brick has the S7 that has undergone mutagenesis and is functional.
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===Source===
 
===Source===
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===References===
 
===References===
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1) NCBI no. 57634611 (S7 nuclease sequence)</br>
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2) Dingwall C, Lomonossoff GP, Laskey RA. High sequence specificity of micrococcal nuclease. Nucleic Acids Res 1981 Jun 25;9(12):2659-2673.

Latest revision as of 03:30, 4 October 2012

S7 micrococcal nuclease


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The S7 nuclease was synthesized from IDT however there was a point mutation in the sequence that hindered the activity of the nuclease. Instead of the 'A' there was a 'T'. We did mutagenesis to change the mutation back to its original nucleotide. This brick has the S7 that has undergone mutagenesis and is functional.

Source

This part was synthesized from IDT and is native to Staphylococcus aureus

References

1) NCBI no. 57634611 (S7 nuclease sequence)</br>

2) Dingwall C, Lomonossoff GP, Laskey RA. High sequence specificity of micrococcal nuclease. Nucleic Acids Res 1981 Jun 25;9(12):2659-2673.