Difference between revisions of "Part:BBa K804010:Experience"
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https://static.igem.org/mediawiki/parts/thumb/d/d3/MetA_complementation_no_fluor.png/800px-MetA_complementation_no_fluor.png | https://static.igem.org/mediawiki/parts/thumb/d/d3/MetA_complementation_no_fluor.png/800px-MetA_complementation_no_fluor.png | ||
− | To generate this graph | + | To generate this graph we cultured the MetA knockout transformed with this part in M9 minimal media with the indicated concentrations of arabinose, and measured the OD600 every 15 minutes. |
- [[User:jacobtoth|Jacob Toth]] | - [[User:jacobtoth|Jacob Toth]] |
Revision as of 03:30, 4 October 2012
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K804010
User Reviews
UNIQ25dfae8fc41af5db-partinfo-00000000-QINU UNIQ25dfae8fc41af5db-partinfo-00000001-QINU
This part consists of an arabinose promoter, a strong RBS, and the metA coding gene. It is able to complement a metA knockout, and the growth rate appears to be proportional to the amount of arabinose that is added.
To generate this graph we cultured the MetA knockout transformed with this part in M9 minimal media with the indicated concentrations of arabinose, and measured the OD600 every 15 minutes.