Difference between revisions of "Part:BBa K804010:Experience"

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https://static.igem.org/mediawiki/parts/thumb/d/d3/MetA_complementation_no_fluor.png/800px-MetA_complementation_no_fluor.png
 
https://static.igem.org/mediawiki/parts/thumb/d/d3/MetA_complementation_no_fluor.png/800px-MetA_complementation_no_fluor.png
  
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To generate this graph, we cultured the metA auxotroph transformed with this part in M9 media and added the described amount of arabinose. The OD600 of the cultures were measured every 15 minutes, and was run overnight.
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To generate this graph we cultured the MetA knockout transformed with this part in M9 minimal media with the indicated concentrations of arabinose, and measured the OD600 every 15 minutes.
  
 
- [[User:jacobtoth|Jacob Toth]]
 
- [[User:jacobtoth|Jacob Toth]]

Revision as of 03:30, 4 October 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K804010

User Reviews

UNIQ3b8b18abb4857489-partinfo-00000000-QINU UNIQ3b8b18abb4857489-partinfo-00000001-QINU

This part consists of an arabinose promoter, a strong RBS, and the metA coding gene. It is able to complement a metA knockout, and the growth rate appears to be proportional to the amount of arabinose that is added. 800px-MetA_complementation_no_fluor.png

To generate this graph we cultured the MetA knockout transformed with this part in M9 minimal media with the indicated concentrations of arabinose, and measured the OD600 every 15 minutes.

- Jacob Toth