Difference between revisions of "Part:BBa K804010:Experience"

 
(User Reviews)
Line 1: Line 1:
 
 
__NOTOC__
 
__NOTOC__
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
Line 19: Line 18:
 
<!-- End of the user review template -->
 
<!-- End of the user review template -->
 
<!-- DON'T DELETE --><partinfo>BBa_K804010 EndReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K804010 EndReviews</partinfo>
 +
 +
This part consists of an arabinose promoter, a strong RBS, and the metA coding gene. It is able to complement a metA knockout, and the growth rate appears to be proportional to the amount of arabinose that is added.
 +
https://static.igem.org/mediawiki/parts/thumb/d/d3/MetA_complementation_no_fluor.png/800px-MetA_complementation_no_fluor.png
 +
 +
To generate this graph, we cultured the metA auxotroph transformed with this part in M9 media and added the described amount of arabinose. The OD600 of the cultures were measured every 15 minutes, and was run overnight.
 +
 +
- [[User:jacobtoth|Jacob Toth]]

Revision as of 03:27, 4 October 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K804010

User Reviews

UNIQd4334a06782e2454-partinfo-00000000-QINU UNIQd4334a06782e2454-partinfo-00000001-QINU

This part consists of an arabinose promoter, a strong RBS, and the metA coding gene. It is able to complement a metA knockout, and the growth rate appears to be proportional to the amount of arabinose that is added. 800px-MetA_complementation_no_fluor.png

To generate this graph, we cultured the metA auxotroph transformed with this part in M9 media and added the described amount of arabinose. The OD600 of the cultures were measured every 15 minutes, and was run overnight.

- Jacob Toth