Difference between revisions of "Part:BBa K206009:Experience"

 
 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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<I>Alex273</I>
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The plasmid seems to be missing the X and S restriction sites. Digestion by these enzymes fails
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[[Image:GelK206009.jpg]]
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''Agarose electrophoresis of part K206009 as ordered from the registry in spring 2011 digested with several iGEM enzymes. nd stands for "non digested"''
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As shown above, the digestion by P linearizes the plasmid as expected but X and S have no effect compared to the non digested plasmid. Moreover, double digestion by X+P only linearizes the plasmid.
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<I>Himika9</I>
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<html><p> The kit plate DNA of this part from Spring 2012 distribution did not work in the hands of the 2012 iGEM Calgary part. University of Calgary 2012 iGEM team built BBa_B0034 and BBa_K082003 and resubmitted this part as <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K902016">BBa_K902016</a>.</html>
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Latest revision as of 02:52, 4 October 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K206009

User Reviews

UNIQdb4474b965641202-partinfo-00000000-QINU UNIQdb4474b965641202-partinfo-00000001-QINU

Alex273

The plasmid seems to be missing the X and S restriction sites. Digestion by these enzymes fails GelK206009.jpg

Agarose electrophoresis of part K206009 as ordered from the registry in spring 2011 digested with several iGEM enzymes. nd stands for "non digested"

As shown above, the digestion by P linearizes the plasmid as expected but X and S have no effect compared to the non digested plasmid. Moreover, double digestion by X+P only linearizes the plasmid.

Himika9

The kit plate DNA of this part from Spring 2012 distribution did not work in the hands of the 2012 iGEM Calgary part. University of Calgary 2012 iGEM team built BBa_B0034 and BBa_K082003 and resubmitted this part as BBa_K902016.