Difference between revisions of "Part:BBa K902050:Design"
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===Source=== | ===Source=== | ||
| − | Obtained from PCR amplification from a plasmid found in an environmental isolate of R. erythropolis shown to be an active desulfurizer. | + | Obtained from PCR amplification from a plasmid found in an environmental isolate of <i>R. erythropolis</i> shown to be an active desulfurizer. |
===References=== | ===References=== | ||
| + | Gou,Z., Li,X., Xing,J., Liu,H. and Chen,J. LSSE8-1 and its desulfurizational genes. Laboratory of Separation Science and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 1# Beiertiao, Zhongguancun, Haidian, Beijing 100080, China. 2003. | ||
Latest revision as of 02:44, 4 October 2012
DBT-sulfone monooxygenase (DszA) from R. erythropolis
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 754
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 43
Illegal NgoMIV site found at 258
Illegal NgoMIV site found at 562
Illegal NgoMIV site found at 682
Illegal AgeI site found at 217 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1066
Design Notes
4 internal PstI cut-sites had to be mutated with site-directed mutagenesis.
Source
Obtained from PCR amplification from a plasmid found in an environmental isolate of R. erythropolis shown to be an active desulfurizer.
References
Gou,Z., Li,X., Xing,J., Liu,H. and Chen,J. LSSE8-1 and its desulfurizational genes. Laboratory of Separation Science and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 1# Beiertiao, Zhongguancun, Haidian, Beijing 100080, China. 2003.