Difference between revisions of "Part:BBa K779501"

(Usage and Biology)
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When annealed to [[Part:BBa K779502]] acts as a reporter for strand displacement reactions.
 
When annealed to [[Part:BBa K779502]] acts as a reporter for strand displacement reactions.
  
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<img src="https://static.igem.org/mediawiki/2012/7/7c/MIT2012_In_vivo_SD_Transfection_FACS_small.png">
  
 
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===Sequence and Features===
 
===Sequence and Features===
  

Revision as of 02:35, 4 October 2012

Status: 500 Content-type: text/html

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For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.


Long reporter top strand to be annealed with Part:BBa K779502.

Modifications: 5' Iowa Black RQ quencher, 3' Alexa 488 florophore, all bases modified with 2'O Methyl (ordered as a DNA oligo with Us instead of Ts).

IDT DNA oligo order: /5IAbRQ/mCmAmCmCmCmAmCmUmCmCmUmCmUmCmCmCmAmCmCmA/3AlexF488N/

Usage and Biology

When annealed to Part:BBa K779502 acts as a reporter for strand displacement reactions.

<img src="MIT2012_In_vivo_SD_Transfection_FACS_small.png">


Sequence and Features

Sequence is designed to be orthogonal to the HEK293 transcriptome (see Design). Design is improved on the part Part:BBa_K779121, with goals being a higher melting temperature, more orthogonal and longer domain.


Status: 500 Content-type: text/html

Software error:

Not enough arguments for Range::range at /websites/parts.igem.org/cgi/lib/Range.pm line 250, near "range;"
Unmatched right curly bracket at /websites/parts.igem.org/cgi/lib/Range.pm line 251, at end of line
syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 251, near "}"
Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 261, near "= @_"
syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 282, near "}"
Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 286, near "= @_"
Global symbol "$course" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 288.
syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 314, near "}"
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16.
Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.

For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.