Difference between revisions of "Part:BBa K844012:Design"
Ryanputman (Talk | contribs) (→Design Notes) |
Ryanputman (Talk | contribs) (→Design Notes) |
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===Design Notes=== | ===Design Notes=== | ||
| − | When working with tRNA expression systems, you cannot have scar sites between the promoter and the gene, or between the gene and the terminator. As such, the entire sequence was synthesized without scars. At minimum, no scars can be internal to an individual tRNA expression unit, but can exist between different complete expression units. Note: the Arg(TCT) tRNA is from Escherichia_coli_O157H7 since the K12 ARG tRNA had an internal PstI site. | + | When working with tRNA expression systems, you cannot have scar sites between the promoter and the gene, or between the gene and the terminator. As such, the entire sequence was synthesized without scars. At minimum, no scars can be internal to an individual tRNA expression unit, but can exist between different complete expression units. Note: All tRNAs are from E. coli K12 except the Arg(TCT) tRNA is from Escherichia_coli_O157H7 since the K12 ARG tRNA had an internal PstI site. |
===Source=== | ===Source=== | ||
Revision as of 02:27, 4 October 2012
tRNA expression cassette for spider silk "F" proteins
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 196
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
When working with tRNA expression systems, you cannot have scar sites between the promoter and the gene, or between the gene and the terminator. As such, the entire sequence was synthesized without scars. At minimum, no scars can be internal to an individual tRNA expression unit, but can exist between different complete expression units. Note: All tRNAs are from E. coli K12 except the Arg(TCT) tRNA is from Escherichia_coli_O157H7 since the K12 ARG tRNA had an internal PstI site.
Source
This part was chemically synthesized but based upon E. coli genomic DNA sequences, and BioBrick parts BBa_K844010 and BBa_K844011.