Difference between revisions of "Part:BBa P1010"
Smelissali (Talk | contribs) (→BioBrick Plasmids Containing This Part) |
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P1010 is used when putting BioBrick parts into BioBrick plasmids. The part to be inserted and the plasmid are cut with BioBrick enzymes and mixed. The mixture will include both the original uncut or religated plasmid and the desired structure. However, because of CcdB, all of the cells containing the original plasmid die and the surviving colonies are the desired result. | P1010 is used when putting BioBrick parts into BioBrick plasmids. The part to be inserted and the plasmid are cut with BioBrick enzymes and mixed. The mixture will include both the original uncut or religated plasmid and the desired structure. However, because of CcdB, all of the cells containing the original plasmid die and the surviving colonies are the desired result. | ||
| − | ===BioBrick Plasmids Containing This Part=== | + | ===BioBrick [[Help:Plasmids/Construction_Plasmids#Construction_Plasmid|Construction Plasmids]] Containing This Part=== |
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| + | Most BioBrick [[Help:Plasmids|plasmids]] are delivered with the P1010 insert | ||
| + | see [https://parts.igem.org/cgi/partsdb/dna.cgi?part_name=P1010 P1010 Physical DNA] for the current list of plasmids that are available in this form. This particular Biobrick with its [[Help:Plasmids/Construction_Plasmids#Plasmid (backbone)|Plasmid backbone]] is considered a | ||
| + | [[Help:Plasmids/Construction_Plasmids#Construction_Plasmid|Construction Plasmid]].<br> | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_P1010 SequenceAndFeatures</partinfo> | <partinfo>BBa_P1010 SequenceAndFeatures</partinfo> | ||
Revision as of 20:04, 9 June 2006
ccdB cell death gene
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The CcdB protein, constitutively expressed by P1010, is lethal to most of the BioBrick cell strains, only DB3.1 is resistant. |
P1010 is used when putting BioBrick parts into BioBrick plasmids. The part to be inserted and the plasmid are cut with BioBrick enzymes and mixed. The mixture will include both the original uncut or religated plasmid and the desired structure. However, because of CcdB, all of the cells containing the original plasmid die and the surviving colonies are the desired result.
BioBrick Construction Plasmids Containing This Part
Most BioBrick plasmids are delivered with the P1010 insert
see P1010 Physical DNA for the current list of plasmids that are available in this form. This particular Biobrick with its Plasmid backbone is considered a
Construction Plasmid.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 664
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 114