Difference between revisions of "Part:BBa K902018:Design"

 
 
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<partinfo>BBa_K902018 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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MgtAp-mgtArb-S7
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<html>This system must be grown on LB containing 10mM+ concentration of MgCl<sub>2</sub> because the system is active at low concentrations of magnesium and will kill the cells, or select for escapers, i.e, mutate out the regulatory elements.</html>
  
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===Source===
  
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MgtAp, mgtArb, and S7 were synthesized. Then these parts were constructed together using the Biobrick assembly method.
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===References===
  
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===Source===
 
  
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MgtAp-mgtArb-S7
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Michael J. Cromie and Eduardo A. Groisman. Promoter and Riboswitch Control of the Mg2+ Transporter MgtA from Salmonella enterica. J Bacteriol. 2010 January; 192(2): 604–607.
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===References===
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Latest revision as of 02:14, 4 October 2012

MgtAp-mgtArb-S7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This system must be grown on LB containing 10mM+ concentration of MgCl2 because the system is active at low concentrations of magnesium and will kill the cells, or select for escapers, i.e, mutate out the regulatory elements.

Source

MgtAp, mgtArb, and S7 were synthesized. Then these parts were constructed together using the Biobrick assembly method.

References

Michael J. Cromie and Eduardo A. Groisman. Promoter and Riboswitch Control of the Mg2+ Transporter MgtA from Salmonella enterica. J Bacteriol. 2010 January; 192(2): 604–607.