Difference between revisions of "Part:BBa K902035:Design"
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<partinfo>BBa_K902035 short</partinfo> | <partinfo>BBa_K902035 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | + | Nucleotide 72 was mutated from a G in the native organism to an A in our construct. This causes a silent mutation which does not affect the amino acid sequence. | |
| − | + | ||
===Source=== | ===Source=== | ||
| − | + | Gene was amplified using PCR from a Rhodococcus erythropolis strain 417 genomic prep template. | |
===References=== | ===References=== | ||
| + | |||
| + | Pavlova,J. and Maksimov,A. The ability of Rhodococcus erythropolis to transform amides. Laboratory of Chemical Mutagenesis,Institute of Ecology and Genetics of Microorganisms, Ural Branch of RAS, 13 Golev Street, Perm 614000, Russia. 2011 | ||
Latest revision as of 02:00, 4 October 2012
AmdA Coding Sequence
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 590
Illegal XhoI site found at 82 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 90
Illegal NgoMIV site found at 1295
Illegal NgoMIV site found at 1401
Illegal AgeI site found at 468
Illegal AgeI site found at 652 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 326
Illegal BsaI site found at 867
Design Notes
Nucleotide 72 was mutated from a G in the native organism to an A in our construct. This causes a silent mutation which does not affect the amino acid sequence.
Source
Gene was amplified using PCR from a Rhodococcus erythropolis strain 417 genomic prep template.
References
Pavlova,J. and Maksimov,A. The ability of Rhodococcus erythropolis to transform amides. Laboratory of Chemical Mutagenesis,Institute of Ecology and Genetics of Microorganisms, Ural Branch of RAS, 13 Golev Street, Perm 614000, Russia. 2011