Difference between revisions of "Part:BBa K902032:Design"

 
 
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__NOTOC__
 
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<partinfo>BBa_K902032 short</partinfo>
 
<partinfo>BBa_K902032 short</partinfo>
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===Design Notes===
 
===Design Notes===
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none
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Source gene has a GTG start codon rather than an ATG start codon. Since the A in an ATG start codon is required to complete the biobrick XbaI site, this was mutated in our construct.
  
  
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===Source===
 
===Source===
  
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tbd
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Gene was amplified by PCR using <i>Pseudomonas putida</i> plasmid pCAR1.2 as a template
  
 
===References===
 
===References===
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Shintani,M., Yoshida,T., Habe,H., Omori,T. and Nojiri,H. Large plasmid pCAR2 and class II transposon Tn4676 are functional mobile genetic elements to distribute the carbazole/dioxin-degradative car gene cluster in different bacteria. Appl. Microbiol. Biotechnol. 67 (3), 370-382 (2005).

Latest revision as of 01:57, 4 October 2012

CarAa Coding Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source gene has a GTG start codon rather than an ATG start codon. Since the A in an ATG start codon is required to complete the biobrick XbaI site, this was mutated in our construct.


Source

Gene was amplified by PCR using Pseudomonas putida plasmid pCAR1.2 as a template

References

Shintani,M., Yoshida,T., Habe,H., Omori,T. and Nojiri,H. Large plasmid pCAR2 and class II transposon Tn4676 are functional mobile genetic elements to distribute the carbazole/dioxin-degradative car gene cluster in different bacteria. Appl. Microbiol. Biotechnol. 67 (3), 370-382 (2005).