Difference between revisions of "Part:BBa K880005"

Revision as of 01:45, 4 October 2012

Strong promoter, strong RBS combination for high expression levels of proteins

The part is composed of sub-parts J23100 and B0034.

This is a strong promoter, strong RBS combination for high expression levels of proteins. It has a consensus constitutive promoter and RBS sequence-produces strongest possible expression.

Use of this Part by Team Michigan '12

Team Michigan '12 used this part as their primary protein-generator. Our data suggests that we were successful in using it to express FimE and HBiF recombinases for our recombinase-based switch systems. See our results page for more information[http://2012.igem.org/Team:Michigan/Results].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K880005 short</partinfo>
--J23100+B0034
+The part is composed of sub-parts J23100 and B0034.
--Strong promoter, strong RBS combination for high expression levels of proteins
-Consensus constitutive promoter and RBS sequence-produces strongest possible expression.
+This is a strong promoter, strong RBS combination for high expression levels of proteins. It has a consensus constitutive promoter and RBS sequence-produces strongest possible expression.
+<h2>Use of this Part by Team Michigan '12</h2>
+Team Michigan '12 used this part as their primary protein-generator. Our data suggests that we were successful in using it to express FimE and HBiF recombinases for our recombinase-based switch systems. See our results page for more information[http://2012.igem.org/Team:Michigan/Results].
 <!-- Add more about the biology of this part here