Difference between revisions of "Part:BBa K900001:Design"
(→‎Source)
Line 12: Line 12:
 
===Source===
 
===Source===
  
−
Synthesized in house and cloned into addgene plasmid l11911 Previously engineered version of RFP from Patterson and Lippincott-Schwartz, 2002.  
+
Synthesized in house and cloned into Addgene plasmid [http://www.addgene.org/11911/ l11911]  Previously engineered version of RFP from Patterson and Lippincott-Schwartz, 2002.
  
 
===References===
 
===References===
  
 
Patterson, G. H. & Lippincott-Schwartz, J. A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells. Science 297, 1873–1877 (2002).
 
Patterson, G. H. & Lippincott-Schwartz, J. A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells. Science 297, 1873–1877 (2002).

Revision as of 01:36, 4 October 2012

PAGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization for yeast and internal restriction site removal to be compatible with our Golden Gate cloning scheme.


Source

Synthesized in house and cloned into Addgene plasmid [http://www.addgene.org/11911/ l11911] Previously engineered version of RFP from Patterson and Lippincott-Schwartz, 2002.

References

Patterson, G. H. & Lippincott-Schwartz, J. A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells. Science 297, 1873–1877 (2002).