Difference between revisions of "Part:BBa K842007"
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CheB removes the methyl group that are attached to the gamma-glutamyl methyl ester residues located in the methyl-accepting chemotaxis protein (MCP). This allows for the bacteria to remain alert to the changes in the environment. | CheB removes the methyl group that are attached to the gamma-glutamyl methyl ester residues located in the methyl-accepting chemotaxis protein (MCP). This allows for the bacteria to remain alert to the changes in the environment. | ||
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Cloning and Uses | Cloning and Uses | ||
This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. It is to be used with other chemotaxis genes to form a complete regulatory pathway that controls the response system in MCP and the direction of rotation in flagella. | This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. It is to be used with other chemotaxis genes to form a complete regulatory pathway that controls the response system in MCP and the direction of rotation in flagella. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 01:16, 4 October 2012
cheB
CheB removes the methyl group that are attached to the gamma-glutamyl methyl ester residues located in the methyl-accepting chemotaxis protein (MCP). This allows for the bacteria to remain alert to the changes in the environment.
Used for researcher control of signaling mechanisms that influence flagella function.
Cloning and Uses
This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. It is to be used with other chemotaxis genes to form a complete regulatory pathway that controls the response system in MCP and the direction of rotation in flagella.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 841
- 1000COMPATIBLE WITH RFC[1000]