Difference between revisions of "Part:BBa J45004:Design"
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Designed Forward Strand (1 and 2): | Designed Forward Strand (1 and 2): | ||
| − | 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA | + | 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G <b>ATG GAA GTT GTT GAA GTT C</b> -3'<br> |
*LENGTH: 19 <br> | *LENGTH: 19 <br> | ||
*GC CONTENT: 36.8 % <br> | *GC CONTENT: 36.8 % <br> | ||
Revision as of 16:02, 9 June 2006
SAM:benzoic acid/salicylic acid carboxyl methyltransferase I; converts salicylic acid to methyl sali
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 663
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 204
Design Notes
Just CDS taken from GenBank
Designed Forward Strand (1 and 2):
5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G ATG GAA GTT GTT GAA GTT C -3'
- LENGTH: 19
- GC CONTENT: 36.8 %
- MELT TEMP: 47.8 ºC
Designed Reverse Strand (phBSMT1): 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AAG GAG -3'
- LENGTH: 22
- GC CONTENT: 27.3 %
- MELT TEMP: 46.6 ºC
Source
Species: Petunia x hybrida from GenBank (AY233465); (also available: A. thaliana and N. suaveolens)
References
- <biblio>Underwood05 pmid=15849311</biblio>
- <biblio>Negre03 pmid=14630969</biblio>