Difference between revisions of "Part:BBa K842005"
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FliH functions as part of the flagella specific export system and is necessary for flageller growth and assembly. It is believed to be part of a two-component system consisting of fliH and fliL that hydrolyses ATP until construction of the flagella apparatus is completed. | FliH functions as part of the flagella specific export system and is necessary for flageller growth and assembly. It is believed to be part of a two-component system consisting of fliH and fliL that hydrolyses ATP until construction of the flagella apparatus is completed. | ||
Revision as of 01:09, 4 October 2012
fliH
FliH functions as part of the flagella specific export system and is necessary for flageller growth and assembly. It is believed to be part of a two-component system consisting of fliH and fliL that hydrolyses ATP until construction of the flagella apparatus is completed.
Used for reconstitution of flagella apparatus in E. coli B strains.
Cloning and Uses: This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. It is to be used in conjunction with the entire flagella synthesis genetic pathway to reproduce a complete flagella apparatus.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]