Difference between revisions of "Part:BBa K847060:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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'''Assay'''<BR>
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how you used this part and how it worked out.
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Liquid cultures of negative control, MntH, betAB, and otsAB transformed NEB5α ''E. coli'' were grown up over night at 37°C. After incubation, a dilution spot assay was conducted on each of the cultures to determine the density of live cells. Next, 15 5cm, round petri dishes were filled with 300uL of negative control bacteria, another 15 with transformants containing MntH, another 15 with transformants containing betAB, and another 15 with transformants containing otsAB. The petri dishes were allowed to desiccate while covered for 24 hours at 37°C while shaking. After the 24 hours period, each plate was resuspended with 1mL of fresh LB. A dilution spot assay was then conducted on each of the petri dish to determine the final density of live cells. <BR><BR>
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'''Results'''<BR>
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Works. Refer to: http://2012.igem.org/Team:Stanford-Brown/HellCell/Desiccation
  
 
===Applications of BBa_K847060===
 
===Applications of BBa_K847060===

Revision as of 00:53, 4 October 2012

Assay
Liquid cultures of negative control, MntH, betAB, and otsAB transformed NEB5α E. coli were grown up over night at 37°C. After incubation, a dilution spot assay was conducted on each of the cultures to determine the density of live cells. Next, 15 5cm, round petri dishes were filled with 300uL of negative control bacteria, another 15 with transformants containing MntH, another 15 with transformants containing betAB, and another 15 with transformants containing otsAB. The petri dishes were allowed to desiccate while covered for 24 hours at 37°C while shaking. After the 24 hours period, each plate was resuspended with 1mL of fresh LB. A dilution spot assay was then conducted on each of the petri dish to determine the final density of live cells.

Results
Works. Refer to: http://2012.igem.org/Team:Stanford-Brown/HellCell/Desiccation

Applications of BBa_K847060

User Reviews

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